Figure 5. Mutation analyses on the target MCM7 of EGR1.

This figure depicts the effect of a mutation in each EGR1 binding site cluster of the MCM7 promoter on the expression level of MCM7, which is a target of the TF EGR1. The strongest binding site in each cluster were abolished by a single nucleotide variant. Upon loss of all three clusters, only weak binding sites remained and EGR1 was predicted to no longer be able to effectively regulate MCM7 expression. Multiple clusters in the promoters of TF targets confer robustness against mutations within individual binding sites that define these clusters.