Table 4.

Novel rat E. coli genomes have similar statistics as pathogenic, PKS-encoding E. coli strains IHE3034 and NC101

Strain	Isolation source	Genome length (bp)	Contigs	G+C% content	Protein-coding sequences	tRNA	rRNA	Virulence factor genes	GenBank accession no.
S11	Research rat	5201802	32	49.72	5149	81	10	cdtABC, gad, iroN, iss, mchB, mchC, mchF, mcmA, pic, PKS, vat	NHYT00000000
S14		5208467	37	49.64	5153	81	10	cdtABC, gad, iroN, iss, mchB, mchC, mchF, mcmA, pic, PKS, vat	NHYQ00000000
S12		5092914	14	50.04	4995	77	11	iroN, iss, PKS, sfaS, vat	NHYS00000000
S13		5296109	47	48.57	5101	73	9	astA, cba, cma, gad, lpfA, pic, PKS	NHYR00000000
S15		5248403	58	47.71	5078	79	8	celb, gad, iss, PKS	NHYP00000000
S16		5623575	140	45.33	5509	70	3	cdtABC, celb, gad, iss, PKS	NHYO00000000
S17		5139109	40	49.47	5022	76	9	cnf1, gad, iroN, mchB, mchC, mchF, mcmA, PKS, vat	QLVH00000000
IHE3034	Human neonatal meningitis	5108383	1 (complete genome)	50.70	5045	97	22	gad, iroN, iss, PKS, sfaS, vat, cdtABC	CP001969.1
NC101	Research mouse	5021144	27	50.57	4917	72	4	gad, iroN, iss, PKS, sfaS, vat	AEFA00000000.1
K12 substrain DH10B	Human nonpathogenic	4686137	1 (complete genome)	50.80	4606	87	14	gad, iss	CP000948.1

astA, EAST-1 heat-stable toxin; cba, colicin B; cdtABC, cytolethal distending toxin subunits A, B, and C; celb, endonuclease colicin E2; cma, colicin M; gad, glutamate decarboxylase; iroN, enterobactin siderophore receptor protein; iss, increased serum survival; lpfA, long polar fimbriae; mchB, microcin H47 part of colicin H; mchC, MchC protein; mchF, ABC transporter protein MchF; mcmA, microcin M part of colicin H; pic, serine protease autotransporters of Enterobacteriaceae (SPATE); PKS, polyketide synthetase (colibactin); sfaS, S-fimbriae minor subunit; vat, vacuolating autotransporter toxin

Virulence factor genes for toxins, survival factors, and adhesions were identified in the rat E. coli genomes.