KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal (clone AC-74) anti-mouse β-Actin (Used at 1:5000) | Sigma Aldrich | RRID AB_476697, Cat# A2228 |
| Polyclonal rabbit anti-mouse Dlk1 (Used at 1:250) | Santa Cruz Biotechnology | RRID AB_2292943, Cat# sc-25437 |
| Monoclonal (D3B8) rabbit anti-mouse cleaved Notch1 (Val1744) (Used at 1:2000) | Cell Signaling Technology | RRID AB_2153348, Cat# 4147 |
| Polyclonal rabbit anti-mouse pro-Sp-C (Used at 1:500) | Millipore | RRID AB_91588, Cat# AB3786 |
| Polyclonal rabbit anti-Histone H3, phosphor (Ser10) (Used at 1:2000) | Millipore | RRID AB_310177, Cat# 06–570 |
| Monoclonal hamster (8.1.1) anti-T1 a (Used at 1:50) | Developmental Studies Hybridoma Bank, University of Iowa | RRID AB_531893 |
| Polyclonal rabbit anti-RFP (Used at 1:200) | Rockland Immunochemicals | RRID AB_11182807, Cat# 600-401-379S |
| Polyclonal chicken anti-GFP (Used at 1:500) | Aves Laboratories | RRID AB_10000240, Cat# GFP-1020 |
| Rat APC anti-mouse EpCAM clone G8.8 (Used at 1:100) | BioLegend | RRID AB_1134102, Cat# 118214 |
| Rat IgG2a, kappa clone RTK2758 (Used at 1:100) | BioLegend | RRID not listed, Cat # 400512 |
| Monoclonal rat anti-mouse CD16/32 clone 93 (used at 0.042mg/ml) | Biolegend | RRID AB_312801, Cat#101302 |
| Monoclonal rat anti-mouse CD45 clone 30-F11 (used at 0.016mg/ml) | BioLegend | RRID AB_312967, Cat# 103102 |
| Rabbit anti CC10 polyclonal antibody, used 1:500 | Fisher | Cat# 07623MI |
| Anti-GAPDH mouse monoclonal antibody, used 1:5000 | Sigma Aldrich | RRID AB_1078991, Cat# G8795 |
| Rabbit anti Ki67 | AbCam | RRID AB_443209, Cat# ab15580 |
| Bacterial and Virus Strains | ||
| Pseudomonas aeruginosa P103 | From Dr. Ruxana Sadikot, Emory University | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma Aldrich | Cat#T5648 |
| Dispase | Corning | Cat#354235 |
| TriZol | Thermo Fisher | Cat#15596018 |
| DNase I | Sigma Aldrich | Cat#D4263 |
| Fetal Bovine Serum | Sigma Aldrich | Cat#12306C |
| HEPES | Life Technology | Cat# 15630080 |
| Penicillin/streptomycin | GIBCO | Cat# 15140122 |
| Gentamicin/Amphotericin | Lonza | Cat# CC-4083 |
| DMEM | Corning Cellgro | Cat#10–013-CV |
| RIPA buffer | Cell Signaling Technology | Cat#9803 |
| cOmplete Protease Inhibitor Cocktail | Roche | Cat#11-836-145-001 |
| Red Blood Cell Lysis Buffer | BioLegend | Cat#420301 |
| Bovine Serum Albumin | MP Biomedicals | Cat#180549 |
| Normal Donkey Serum | Jackson Immunoresearch | Cat# 017-000-12 |
| Paraformaldehyde | Sigma Aldrich | Cat#P6148 |
| SYBR Green | Roche | Cat#04913914001 |
| SuperSignal West Pico Chemiluminescent Substrate | Thermo Scientific | Cat#34080 |
| SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Scientific | Cat#34095 |
| Vectashield Antifade Mounting Medium | Vector Laboratories | Cat# H-1000 |
| Critical Commercial Assays | ||
| RNeasy Kit | QIAGEN | Cat#74104 |
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat#4368814 |
| Micro BCA Protein Assay Kit | Thermo Scientific | Cat#23235 |
| in situ Cell Death Kit TMR | Sigma Aldrich | Cat#12156792910 |
| Deposited Data | ||
| RNA sequencing | NCBI Gene Expression Omnibus (GEO) database | Accession Number: GSE 124259 |
| Experimental Models: Organisms/Strains | ||
| Mouse: SpC-CreER: Sfptctm(cre/ERT2>Blh | Brigid Hogan, Duke University | N/A |
| Mouse: ROSA-ICN | Dr. Ben Stanger, University of Pennsylvania | N/A |
| Mouse: Dlk1flox: Dlk1tm1.1Jvs/J | The Jackson Laboratory | IMSR Cat# JAX:019074 |
| Mouse: Rosa-tomato:B6.Cg-Gt(ROSA)26Sortm9(CAG 81 tdTomato)Hze/J | The Jackson Laboratory | Stock No: 007914 |
| Mouse: Notch-Venus: Tg(Cp-HIST1H2BB/Venus)47Hadj/J | The Jackson Laboratory | IMSR Cat# JAX:020942 |
| Mouse: ROSA-dnMAML: | Dr. Warren Pear, University of Pennsylvania | N/A |
| Mouse: Rosa-YFP: B6.129X1-Gt(ROSA) 26Sortm 1(EYFP)Cos/J | The Jackson Laboratory | IMSR Cat# JAX:006148 |
| Mouse: C57BL/6 | The Jackson Laboratory | Stock No: 0664 |
| Oligonucleotides | ||
| Quantitative real-time PCR Primers | Table S1 | IDT or QIAGEN |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| ZEN v2.3 | Zeiss Microscopy | N/A |
| AxioVision LE | Zeiss Microscopy | N/A |
| QuantStudio v1.3 | Applied Biosystems | N/A |
| Summit (v4.3) | Beckman Coulter | N/A |
| Excel | Microsoft | N/A |
| Other | ||
| Western blot precast gels 4–20% SDS-PAGE gels | BioRad | Cat#456–1094 |
| PVDF | BioRad | Cat# 162–0177 |
| 20 μm nylon mesh | Small Parts | Cat#7050-1220-000-10 |
| Blood agar plates | Medex | Cat#HL-1160 |