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. 2019 Apr 12;2(2):e201800270. doi: 10.26508/lsa.201800270

Figure S1. Supporting data for Fig 1.

Figure S1.

(A) Western blot of STN1 levels in HCT116 cell lines. Actinin was used as a loading control. (B) IF was performed for MCM6 in pre-extracted HCT116 cells. Dot plots of mean MCM6 intensity per nuclei. Black line and numbers below the graph indicate the mean AFU. Error bars represent the ±SEM of three independent experiments. n indicates the number of total nuclei scored. (C) Western blots of MCM levels in HeLa whole cell lysates, as indicated. Ponceau S stain was used as a loading control. (D) IF was performed for MCM6 in pre-extracted HeLa cells. Dot plots of mean MCM6 intensity per nuclei in indicated cell lines. Black line and numbers below the graph indicate the mean AFU. Error bars represent the ±SEM of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test (****P ≤ 0.0001).