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Replicate experiment of Fig 4B. Co-IP assay of lysates from HEK 293T cells expressing Flag-tagged MCM subunits, CDT1-HA together with the CST complex (+CST) or mock plasmids. Lysate from transfected HEK 293T cells were subjected to IP with Flag antibody. Relative CDT1-HA band intensity was then compared ±CST, as indicated below the gels (see the Material and Methods section).
