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. 2019 Apr 15;14(4):e0215215. doi: 10.1371/journal.pone.0215215

Fig 1. GM130 is required for normal Golgi organization in RPE-1 cells.

Fig 1

A. Clonal GM130 knockout (KO) cell lines were generated by targeting Cas9 to exon 1 (KO60) or exon 5 (KO2) of GOLGA2, the gene encoding for GM130. B. Western blotting with an antibody to the GM130 N-terminus (left panel) or C-terminus (right panel). GAPDH served as a loading control. C—E. Wild-type and GM130 KO cells were analyzed by immunofluorescence microscopy with antibodies to GM130 and Giantin (C.) GRASP65 and Giantin (D.) and Mannosidase II and Golgin-97 (E.). For each panel, merged images are also shown in which DNA is stained with DAPI. Scale 10μm.