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. 2019 Apr 15;14(4):e0215072. doi: 10.1371/journal.pone.0215072

Fig 2. Expression of HIF-1 target genes under isoflurane.

Fig 2

(A-D) RCC4-EV and RCC4-VHL cells were exposed for 8 h to 20% or 1% O2 with or without 2% isoflurane. Cells were then harvested, and mRNA levels quantified by semi-quantitative RT-PCR analysis. Relative expression fold-changes were determined from mRNA expression in RCC4-EV cells at 20% O2. Data represent the mean ± SD values (n = 3). *, p < 0.05 vs. cells at 20% O2 and no isoflurane; #, p < 0.05 for the indicated comparison; NS, not significant; SLC2A1, solute carrier family 2 member 1; VEGFA, vascular endothelial growth factor A; HIF1A, hypoxia-inducible factor 1 α subunit; EPAS1, endothelial PAS domain protein 1.