Table 1.

Sequences of the primers and molecular beacon used in RT-PCR and real-time NASBA assays for detecting ASSVd in apple trees

Primer and probe		Sequence (5′→3′)
RT-PCRa	ASSVd-F	CCCGGTAAACACCGTGCGGT
	ASSVd-R	ACCGGGAAACACCTATTGTG
Real-time NASBA	ASSVd-P1	AATTCTAATACGACTCACTATAGGGAAAGAGCGTGAGAGAACAGG
	ASSVd-P2	GCAGATAGATAAAGAAAACGAGGA
	ASSVd-MB	[6-FAM]-CGCGATCaaggagctgccagcactaagccgGATCGCG-[DABCYL]

^aThe primers for RT-PCR were reported by Lee et al. (2001).

^*Underlined letters indicate the T7 RNA polymerse promoter-binding region. Lowercase letters are the specific binding sequences to ASSVd RNA.