Table 1. Steroid content of testis, prostate, and liver from puberal and postpuberal littermate boars treated with the aromatase inhibitor, letrozole, or with the canola oil vehicle1.
| Testis2 | ||||||
| C18 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Estrone | 22.07 (9.92) | 1.96 (0.78) | 17.03 (8.97) | 1.88 (0.49) | 9.96 (2.71) | 4.48 (2.28) |
| Estrone-3-SO4 | 76.11 (29.56) | 0.92 (0.45) | 50.75 (35.24) | 2.02 (2.44) | 49.52 (19.75) | 46.42 (35.49) |
| Estrone-3-GLU | ND | ND | ND | ND | ND | ND |
| 16α-Hydroxyestrone | 1.70 (0.68) | 0.04 (0.04) | 0.68 (0.08) | 0.13 (0.11) | 0.51 (0.31) | 0.33 (0.09) |
| Estradiol | 44.45 (29.11) | 7.88 (3.74) | 56.26 (27.04) | 7.86 (4.62) | 39.59 (4.83) | 19.68 (10.45) |
| Estradiol-3-SO4 | 586.5 (246.2) | 2.69 (1.14) | 381.6 (271.3) | 8.30 (8.91) | 273.9 (70.6) | 199.7 (129.9) |
| Estradiol-3-GLU | 0.27 (0.26) | 0.12 (0.18) | 0.31 (0.62) | 0.03 (0.06) | 0.02 (0.04) | 0.02 (0.03) |
| Estradiol-17-GLU | 3.64 (3.30) | 0.66 (0.77) | 2.94 (2.18) | 0.97 (1.08) | 2.13 (2.13) | 0.24 (0.38) |
| Estriol | 0.20 (0.29) | trace | 0.29 (0.41) | 0.01 (0.02) | 0.04 (0.08) | 0.04 (0.08) |
| C19 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Dehydroepiandrosterone | 85.96 (23.65) | 132.90 (138.80) | 98.91 (105.92) | 48.55 (45.99) | 52.26 (30.66) | 37.13 (32.51) |
| Dehydroepiandrosterone-SO4 | 237.8 (43.87) | 163.7 (179.6) | 193.7 (71.79) | 125.9 (70.36) | 98.37 (15.06) | 102.0 (55.65) |
| 5-Androstenediol | 75.40 (35.83) | 104.3 (107.7) | 86.29 (113.7) | 37.4 (30.84) | 84.27 (88.43) | 28.20 (22.01) |
| 4-Androstenediol | 5.58 (4.64) | 5.01 (4.81) | 5.35 (6.13) | 1.89 (1.21) | 4.77 (3.41) | 2.20 (1.87) |
| Androstenedione | 58.11 (31.33) | 36.20 (32.91) | 53.49 (32.36) | 21.24 (9.45) | 18.20 (12.55) | 13.08 (4.79) |
| 19-Hydroxyandrostendione | 12.80 (14.66) | 0.35 (0.34) | 10.60 (11.26) | 0.93 (0.99) | 2.61 (1.42) | 3.07 (2.78) |
| Testosterone | 236.2 (130.6) | 157.8 (192.4) | 166.5 (92.85) | 84.42 (59.00) | 61.86 (36.59) | 36.84 (12.43) |
| Epitestosterone | 4.50 (2.95) | 2.42 (3.65) | 4.81 (2.94) | 2.32 (0.76) | 1.01 (0.74) | 0.67 (0.49) |
| Epi/Testosterone-SO4 | 12.60 (6.59) | 11.32 (4.70) | 8.44 (6.36) | 4.27 (2.42) | 4.56 (3.92) | 7.53 (6.41) |
| 5α-Dihydrotestosterone | 1.87 (1.88) | 4.39 (6.76) | 2.42 (2.58) | 3.12 (3.31) | 0.54 (0.63) | 1.33 (1.09) |
| 6-Ketotestosterone | 59.83 (96.59) | 55.22 (75.38) | 45.08 (55.70) | 33.28 (27.30) | 7.44 (11.89) | 5.88 (5.80) |
| 6-Dehydrotestosterone | 0.86 (0.46) | 0.40 (0.25) | 0.29 (0.15) | 0.30 (0.09) | 0.21 (0.12) | 0.22 (0.13) |
| 17β-Dihydroepiandrosterone | 181.7 (162.6) | 174.8 (242.7) | 151.0 (170.3) | 98.98 (46.79) | 130.6 (93.27) | 82.13 (51.50) |
| 6α-Hydroxytestosterone | 12.16 (8.52) | 3.54 (6.53) | 9.13 (6.32) | 2.02 (3.49) | 1.49 (0.90) | 0.88 (0.79) |
| Adrenosterone | 2.74 (2.82) | 2.37 (2.47) | 1.40 (0.89) | 1.49 (1.15) | 0.39 (0.45) | 0.40 (0.29) |
| Epiandrosterone | 12.85 (7.10) | 15.66 (26.94) | 10.91 (7.72) | 10.13 (14.69) | 3.81 (4.12) | 6.51 (5.76) |
| C21 | ||||||
| Pregnenolone | 835.4 (357.8) | 822.5 (462.5) | 739.8 (594.0) | 806.3 (725.1) | 484.4 (107.0) | 397.8 (239.8) |
| Pregnenolone-3-SO4 | 59.3 (11.5) | 130.6 (177.8) | 66.7 (52.8) | 87.7 (77.3) | 39.8 (20.9) | 38.5 (33.6) |
| 17-Hydroxypregnenolone | 396.0 (265.0) | 406.5 (360.5) | 421.4 (507.5) | 395.9 (536.0) | 255.1 (208.4) | 214.1 (147.9) |
| Progesterone | 6.92 (2.26) | 10.30 (6.50) | 9.03 (9.34) | 16.86 (23.74) | 5.08 (4.11) | 4.14 (2.98) |
| 17-Hydroxyprogesterone | 2.52 (1.01) | 4.04 (2.70) | 3.48 (3.44) | 4.68 (4.61) | 2.89 (2.13) | 2.08 (1.08) |
| 11-Deoxycortisol | 2.88 (2.15) | 1.07 (0.72) | 2.25 (1.42) | 0.95 (0.71) | 0.97 (0.55) | 0.49 (0.30) |
| Cortisol | 4.14 (4.13) | 0.69 (0.79) | 3.27 (3.09) | 1.01 (0.99) | 2.10 (1.49) | 2.09 (1.39) |
| Deoxycorticosterone | 1.59 (1.22) | 0.50 (0.43) | 1.35 (1.02) | 0.44 (0.26) | 0.44 (0.35) | 0.27 (0.11) |
| Corticosterone | 0.27 (0.52) | 0.41 (0.43) | 1.02 (1.81) | 0.16 (0.12) | 0.37 (0.27) | 0.42 (0.27) |
| Epiallopregnanolone | 78.13 (62.52) | 120.8 (147.3) | 81.82 (97.05) | 162.6 (179.2) | 81.87 (95.14) | 47.55 (25.79) |
| Allo/Pregnanolone-SO4 | 1.59 (1.36) | 3.78 (3.38) | 0.93 (0.61) | 1.90 (1.84) | 0.42 (0.33) | 0.23 (0.18) |
| Epi/Epiallopregnanolone-SO4 | 2.91 (1.80) | 21.39 (35.92) | 7.94 (11.73) | 14.97 (18.26) | 4.37 (5.82) | 3.15 (3.72) |
| Prostate3 | ||||||
| C18 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Estrone | 3.64 (1.86) | 1.59 (0.51) | 2.14 (0.92) | 2.04 (1.18) | 2.94 (2.15) | 1.65 (0.49) |
| Estrone-3-SO4 | 9.37 (7.78) | 0.36 (0.19) | 5.27 (2.42) | 0.49 (0.12) | 9.32 (8.19) | 4.00 (3.01) |
| Estrone-3-GLU | ND | ND | ND | ND | ND | ND |
| 16α-Hydroxyestrone | ND | ND | ND | ND | ND | ND |
| Estradiol | 18.33 (8.28) | 11.80 (5.35) | 15.50 (10.56) | 13.34 (7.89) | 19.43 (14.06) | 8.04 (1.98) |
| Estradiol-3-SO4 | 7.44 (5.38) | 0.54 (0.42) | 4.28 (1.64) | 0.73 (0.99) | 10.86 (13.35) | 3.25 (1.92) |
| Estradiol-3-GLU | 0.35 (0.57) | 0.002 (0.002) | 0.05 (0.09) | 0.12 (0.17) | 1.31 (0.99) | 0.02 (0.03) |
| Estradiol-17-GLU | 0.42 (0.72) | 0.002 (0.001) | 0.20 (0.40) | 1.51 (2.00) | 1.40 (1.23) | 0.94 (0.86) |
| Estriol | ND | ND | ND | ND | ND | ND |
| C19 | ||||||
| Dehydroepiandrosterone | ND | ND | ND | ND | ND | ND |
| Dehydroepiandrosterone-SO4 | 29.29 (19.57) | 9.89 (7.27) | 15.36 (5.18) | 12.16 (7.53) | 31.03 (24.15) | 13.15 (10.51) |
| 5-Androstenediol | ND | ND | ND | ND | ND | ND |
| 4-Androstenediol | ND | ND | ND | ND | ND | ND |
| Androstenedione | 0.26 (0.10) | 0.24 (0.09) | 0.22 (0.10) | 0.13 (0.06) | 0.38 (0.29) | 0.13 (0.01) |
| 19-Hydroxyandrostendione | ND | ND | ND | ND | ND | ND |
| Testosterone | 1.67 (0.32) | 1.04 (1.67) | 0.36 (0.15) | 0.55 (0.29) | 1.07 (0.76) | 0.33 (0.15) |
| Epitestosterone | 0.01 (0.02) | 0.09 (0.07) | 0.02 (0.02) | 0.09 (0.10) | 0.27 (0.53) | 0.27 (0.28) |
| Epi/Testosterone-SO4 | ND | ND | ND | ND | ND | ND |
| 5α-Dihydrotestosterone | 2.51 (1.10) | 1.79 (1.17) | 1.29 (0.84) | 1.23 (0.39) | 0.93 (0.95) | 0.99 (0.49) |
| 6-Ketotestosterone | ND | ND | ND | ND | ND | ND |
| 6-Dehydrotestosterone | ND | ND | ND | ND | ND | ND |
| 17β-Dihydroepiandrosterone | trace | trace | trace | trace | trace | trace |
| 6α-Hydroxytestosterone | ND | ND | ND | ND | ND | ND |
| Adrenosterone | ND | ND | ND | ND | ND | ND |
| Epiandrosterone | 4.76 (2.82) | 6.24 (5.94) | 6.01 (4.52) | 7.01 (4.62) | 4.41 (3.25) | 3.27 (2.01) |
| C21 | ||||||
| Pregnenolone | ND | ND | ND | ND | ND | ND |
| Pregnenolone-3-SO4 | 0.49 (0.39) | 0.36 (0.29) | 0.42 (0.19) | 1.29 (0.88) | 0.98 (0.80) | 0.62 (0.55) |
| 17-Hydroxypregnenolone | ND | ND | ND | ND | ND | ND |
| Progesterone | 0.70 (0.34) | 0.31 (0.21) | 0.73 (0.86) | 1.64 (2.36) | 0.58 (0.50) | 0.25 (0.20) |
| 17-Hydroxyprogesterone | ND | ND | ND | ND | ND | ND |
| 11-Deoxycortisol | ND | ND | ND | ND | ND | ND |
| Cortisol | 2.85 (1.87) | 0.86 (0.72) | 3.13 (4.05) | 3.39 (3.70) | 3.38 (3.57) | 1.12 (0.69) |
| Deoxycorticosterone | 0.24 (0.36) | 0.11 (0.10) | 0.24 (0.26) | 0.24 (0.25) | 0.07 (0.11) | 0.08 (0.07) |
| Corticosterone | 1.84 (1.38) | 0.36 (0.50) | 0.82 (1.15) | 2.32 (3.12) | 0.26 (0.17) | 0.35 (0.28) |
| Epiallopregnanolone | ND | ND | ND | ND | ND | ND |
| Allo/Pregnanolone-SO4 | ND | ND | ND | ND | ND | ND |
| Epi/Epiallopregnanolone-SO4 | trace | trace | trace | trace | trace | trace |
| Liver4 | ||||||
| C18 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Estrone | 0.22 (0.03) | 0.33 (0.13) | 0.53 (0.20) | 0.41 (0.05) | 0.48 (0.21) | 0.29 (0.12) |
| Estrone-3-SO4 | 4.84 (3.40) | 0.06 (0.04) | 6.02 (1.51) | 0.26 (0.39) | 6.85 (4.79) | 2.18 (1.30) |
| Estrone-3-GLU | 1.06 (0.36) | 0.12 (0.22) | 0.14 (0.13) | 0.36 (0.44) | 1.40 (2.23) | 0.90 (1.01) |
| 16α-Hydroxyestrone | 0.01 (0.003) | 0.03 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.01) | 0.02 (0.004) |
| Estradiol | 1.27 (0.05) | 1.68 (0.84) | 2.89 (2.07) | 1.47 (0.50) | 2.40 (0.80) | 1.43 (1.06) |
| Estradiol-3-SO4 | 14.26 (15.44) | 0.13 (0.08) | 34.31 (12.07) | 1.35 (1.70) | 67.40 (43.92) | 21.61 (14.41) |
| Estradiol-3-GLU | 1.19 (1.33) | 0.004 (0.01) | 0.95 (0.88) | 0.02 (0.02) | 3.05 (3.03) | 2.18 (1.72) |
| Estradiol-17-GLU | 1.11 (1.57) | 0.32 (0.64) | 0.57 (0.47) | 0.13 (0.15) | 1.79 (0.29) | 0.62 (0.67) |
| Estriol | 0.07 (0.10) | trace | 0.16 (0.23) | trace | 0.01 (0.02) | 0.21 (0.34) |
| C19 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Dehydroepiandrosterone | ND | ND | ND | ND | ND | ND |
| Dehydroepiandrosterone-SO4 | 11.14 (4.38) | 6.96 (3.49) | 16.70 (4.33) | 11.66 (10.59) | 17.78 (14.61) | 9.23 (6.83) |
| 5-Androstenediol | ND | ND | ND | ND | ND | ND |
| 4-Androstenediol | ND | ND | ND | ND | ND | ND |
| Androstenedione | 0.07 (0.01) | 0.13 (0.05) | 0.34 (0.23) | 0.17 (0.08) | 0.11 (0.09) | 0.07 (0.04) |
| 19-Hydroxyandrostendione | ND | ND | ND | ND | ND | ND |
| Testosterone | 0.19 (0.01) | 0.31 (0.27) | 0.43 (0.05) | 0.65 (0.32) | 0.60 (0.49) | 0.31 (0.08) |
| Epitestosterone | 0.26 (0.08) | 0.18 (0.15) | 0.14 (0.11) | 0.25 (0.23) | 0.20 (0.21) | 0.19 (0.19) |
| Epi/Testosterone-SO4 | 2.35 (2.00) | 2.15 (2.10) | 2.87 (0.76) | 2.90 (3.31) | 4.93 (2.03) | 4.79 (4.31) |
| 5α-Dihydrotestosterone | 0.04 (0.06) | 0.09 (0.10) | 0.04 (0.07) | 0.01 (0.02) | 0.13 (0.15) | 0.05 (0.07) |
| 6-Ketotestosterone | ND | ND | ND | ND | ND | ND |
| 6-Dehydrotestosterone | ND | ND | ND | ND | ND | ND |
| 17β-Dihydroepiandrosterone | ND | ND | ND | ND | ND | ND |
| 6α-Hydroxytestosterone | ND | ND | ND | ND | ND | ND |
| Adrenosterone | ND | ND | ND | ND | ND | ND |
| Epiandrosterone | ND | ND | ND | ND | ND | ND |
| C21 | V-16 | L-16 | V-20 | L-20 | V-40 | L-40 |
| Pregnenolone | ND | ND | ND | ND | ND | ND |
| Pregnenolone-3-SO4 | 0.60 (0.02) | 0.71 (0.86) | 0.87 (0.54) | 1.07 (0.64) | 0.95 (0.39) | 0.96 (0.76) |
| 17-Hydroxypregnenolone | ND | ND | ND | ND | ND | ND |
| Progesterone | 0.06 (0.03) | 0.17 (0.11) | 1.09 (1.16) | 1.05 (1.09) | 0.30 (0.06) | 0.30 (0.14) |
| 17-Hydroxyprogesterone | ND | ND | ND | ND | ND | ND |
| 11-Deoxycortisol | 0.14 (0.08) | 0.28 (0.25) | 2.04 (2.09) | 1.87 (1.86) | 0.36 (0.27) | 0.30 (0.19) |
| Cortisol | 0.47 (0.06) | 1.64 (2.25) | 13.23 (12.51) | 7.73 (8.98) | 1.40 (1.14) | 1.70 (1.05) |
| Deoxycorticosterone | 0.03 (0.01) | 0.04 (0.04) | 0.38 (0.33) | 0.36 (0.45) | 0.11 (0.08) | 0.07 (0.05) |
| Corticosterone | 0.20 (0.21) | 0.30 (0.26) | 0.60 (0.54) | 0.86 (0.91) | 0.07 (0.06) | 0.12 (0.11) |
| Epiallopregnanolone | ND | ND | ND | ND | ND | ND |
| Allo/Pregnanolone-SO4 | ND | ND | ND | ND | ND | ND |
| Epi/Epiallopregnanolone-SO4 | trace | trace | trace | trace | trace | trace |
1Values represent means (SD) in ng/g. Values in bold indicate statistically significant differences between letrozole-treated boars and vehicle-treated littermates at the indicated age.
2Values for testis tissue at each age are based upon one boar from each of four litters treated with letrozole from 11 to 16 weeks of age and littermates treated with the canola oil vehicle. All boars came from a total of five litters.
3Values for prostate tissue at each age are based upon one boar from each of four litters treated with letrozole from 11 to 16 weeks of age (three boars treated with vehicle at 16 weeks of age and three boars treated with letrozole at 20 weeks of age) and littermates treated with the canola oil vehicle. Tissues came from same boars that donated testicular tissue.
4Values for liver tissue at each age are based upon one boar from each of four litters treated with letrozole from 11 to 16 weeks of age (three boars treated with vehicle at 16 weeks of age and at 20 weeks of age) and littermates treated with the canola oil vehicle. Tissues came from same boars that donated testicular tissue.