Table 2. List of steroids evaluated in profile.

Steroid	LOD1	Steroid	LOD1
Pregnenolone	1.58	Estradiol-3-hemisuccinate	0.001
17-Hydroxypregnenolone	1.504	Estrone-3-hemisuccinate	0.007
Dehydroepiandrosterone	0.087	Estradiol-17-hemisuccinate	0.268
5-Androstenediol	0.172	Estradiol-3,17-di-hemisuccinate	0.001
Progesterone	0.003	Estriol-3-hemisuccinate	0.064
17-Hydroxyprogesterone	0.007	Allopregnanolone	0.785
11-Deoxycortisol	0.007	Alldihydroprogesterone	1.58
Cortisol	0.068	Allopregnanediol	0.1
11-Deoxycorticosterone	0.015	11α-Hydroxyprogesterone	0.757
Corticosterone	0.072	11β-Hydroxyprogesterone	0.076
Aldosterone	0.693	21-Hydroxypregnanolone	3.008
Androstenedione	0.009	21-Hydroxypregnenolone	3.008
Testosterone	0.009	7α-Hydroxypregnenolone	3.008
5α-Dihydrotestosterone	0.344	2α-Hydroxytestosterone	0.81
Estrone	0.009	20α-Hydroxy-5a-pregnan-3-one	0.785
Estradiol	0.009	20α-Hydroxyprogesterone	0.521
11α-Hydroxyestrone	0.087	17α-20a-Dihydroprogesterone	0.923
11β-Hydroxyestrone	0.873	Pregnenolone-SO4	0.063
11α-Hydroxyestradiol	0.173	Pregnanolone	0.785
11β-Hydroxyestradiol	1.734	Pregnanediol	1.56
9(11)-Dehydroestradiol	0.092	Pregnanedione	1.58
9(11)-Dehydroestrone	0.093	Epiallopregnanolone	1.36
11-Ketoestrone	0.088	17β-Dihydroandrosterone	3.419
Estradiol-3-SO4	0.025	17β-Dihydroepiandrosterone	1.71
Estrone-3-SO4	0.076	7α-Hydroxytestosterone	0.821
Estradiol-17-SO4	0.049	7α-Hydroxyandrostenedione	0.083
Estradiol-3,17-SO4	0.216	6α-Hydroxytestosterone	0.019
3-o-methoxy-estrone	0.009	6β-Hydroxytestosterone	.
Estradiol-3-GLU	1.062	Dehydroepiandrosterone-GLU	3.46
Estrone-3-GLU	0.073	16α-Hydroxydehydroepiandrosterone	8.55
Estradiol-17-GLU	1.062	Testosterone-SO4	0.062
Estrone-3,17-GLU	0.327	Desoxytestosterone	0.544
4-Hydroxyestrone	0.002	Testosterone-GLU	0.166
4-Hydroxyestradiol	0.347	4-Androsten-3α-ol-17-one	2.19
4-o-Methoxy-estrone	0.083	2α-Hydroxyandrostenedione	6.22
4-o-Methoxy-estradiol	3.307	11α-Hydroxyandrostenedione	1.84
4-o-Methoxy-estriol	15.703	6-Ketotestosterone	0.413
4-Hydroxy-estrone-2-SG	0.042	11-Ketotestosterone	0.928
4-Hydroxy-estradiol-2-SG	0.169	9-Dehydrotestosterone	.
4-Hydroxy-estrone-2-CYS	0.025	Allotetrahydrocortexone	.
4-Hydroxy-estradiol-2-CYS	0.614	Adrenosterone	0.227
4-Hydroxy-estrone-2-NAcCYS	0.224	Etiocholanolone	.
4-Hydroxy-estradiol-2-NAcCYS	0.223	4-Androstenediol	0.826
4-Hydroxy-estrone-1-N3Ade	0.012	16α-Ketotestosterone	2.21
4-Hydroxy-estradiol-1- N3Ade	0.024	19-Hydroxyandrostenedione	0.235
4-Hydroxy-estrone-1-N7Gua	0.23	4-Androsten-3,6-17-trione	2.63
4-Hydroxy-estradiol-1- N7Gua	0.229	7β-Hydroxydehydroepiandrosterone	6.24
2-Hydroxyestrone	0.349	7α-Hydroxydehydroepiandrosterone	6.22
2-Hydroxyestradiol	0.347	7-Ketodehydroepiandrosterone	1.12
2-Hydroxy-estrone-1+4-SG	0.423	9-Dehydroepiandrosterone	.
2-Hydroxy-estradiol-1+4-SG	0.421	Cortisone	.
2-Hydroxy-estrone-1+4-Cys	0.247	9-Dehydroprogesterone	5.29
2-Hydroxy-estradiol-1+4-Cys	0.614	11-Dehydrotetrahydrocosticosterone	0.814
2-Hydroxy-estrone-1+4-NAcCys	0.112	Tetrahydrocorticosterone	.
2-Hydroxy-estradiol-1+4-S NAcCys	0.557	6β-Hydroxycorticosterone	0.618
2-Hydroxyestrone-6-N3Ade	0.119	5α-Dihydrocorticosterone	1.66
2-Hydroxyestradiol-6-N3Ade	0.119	5α-Dihydropregnanolone	.
2-Hydroxyestriol	0.008	Tetrahydrocortisol	.
3-Methoxy-2-hydroxy-estrone	0.166	Pregnanetriol	1.31
2-Methoxy-3-hydroxy-estrone	0.008	Allotetrahydrocortisol	.
2-Methoxy-3-hydroxy-estradiol	0.331	16α-Hydroxyprogesterone	2.25
2,3 Dimethoxy-estrone	0.032	6-Dehydrotestosterone	3.27
2,3 Dimethoxy-estradiol	0.079	9(11)-Didehydroepiandrosterone	9.36
6α-Hydroxyestradiol	0.173	11β-Hydroxyepiandrosterone	1.28
6β-Hydroxyestradiol	0.173	16α-Hydroxyepiandrosterone	8.55
6-Ketoestrone	0.088	Androsterone	.
6-Ketoestradiol	0.087	Epiandrosterone	0.67
6-Dehydroestradiol	0.092	7α-Hydroxyandrostenediol	1.38
6-Dehydroestrone	0.019	7β-Hydroxyandrostenediol	1.29
16α-Hydroxyestrone	0.175	5β-Dihydrocorticosterone	.
17-Epiestriol	0.173	4-Pregnen-3b-ol-20-one	8.06
Estriol	0.347	Urocortisone	0.067
16,17-Epiestriol	3.468	5-Androsten-3b,17-diol-16-one	3.94
16-Epiestriol	3.468	19-Hydroxydehydroepiandrosterone	2.8
Estriol-3-SO4	0.271	16α-Hydroxypregnenolone	4.401
Estriol-3-GLU	2.153	5α-Dihydrocortexone	1.62
3-Methoxy-estriol	0.002	Epitestosterone	0.174
16-Keto-17β-estradiol	3.492	5β-Androstanedione	.
16-Ketoestriol	0.331	11β-Hydroxyandrostenedione	.
7-Dehydro-17β-estradiol	3.699	11-Ketoetiocholanolone	.
Equilin	0.019	5α-Pregnan-11a-ol-3,20-dione	2.51
Dihydroequilin-3-SO4	1.342	5α-Epoxypregnenolone	.
Equilin-3-SO4	6.749	Allopregnanetrione	.
Estrone-9-N3-Ade	0.124	11-Ketoprogesterone	3.12
Estradiol-9-N3-Ade	0.247	11-Dehydrocorticosterone	.
Estradiol-3-acetate	0.08	6β-Hydroxycortisol	.
Estrone-3-acetate	0.16	20β-Dihydrocortisone	.
Estradiol-3,17α-di-acetate	0.07	6β-Hydroxycortisone	.
Estradiol-3,17β-di-acetate	1.402	Etiocholanolone-GLU	0.398
2-Hydroxy-estradiol-17-acetate	0.003	Dehydroepiandrosterone-SO4	0.198
6-Ketoestradiol-3,17-di-acetate	0.006	Epitestosterone-SO4	0.181
6-Ketoestradiol-tri-acetate	0.023	Epitestosterone-GLU	0.126
6-Dehydroestradiol-di-acetate	0.141	Pregnanediol-GLU	0.0183
Estriol-3-acetate	0.303	17α-Hydroxypregnanolone-GLU	1.76
Estriol-16-acetate	0.303	Pregnanolone-SO4	0.133
Estriol-16,17-diacetate	0.268	17α,20β-Dihydroxyprogesterone-GLU	0.102
17-Epiestriol triacetate	0.241	Hydrocortisone-21-SO4	.
Estriol-triacetate	0.006	Pregnenolone-GLU	.
16,17-Epiestriol triacetate	0.06	Allopregnanolone-SO4	0.014
16-Epiestriol triacetate	0.121	Epiallopregnanolone-SO4	0.012
Equilin acetate	0.081	Corticosterone-21-SO4	.

¹LOD values are estimates of the limit of detection. Values in italics are based on a limited assessment and no estimates are available for compounds with a period.