Skip to main content
. 2019 Apr 15;14(4):e0215408. doi: 10.1371/journal.pone.0215408

Fig 2. Effect of CTSL on SIgA secretion.

Fig 2

(A) Knockdown or inhibit CTSL: CTSL was partially or almost completely knockdown when 1.5 μg or 2 μg plasmid px458-253r were added in a well of a 24 well plates; 5 μg or 10 μg E64 could inhibit CTSL. (B) Levels of SIgA significantly decreased when DCs+B cells were incubated with 2 μg plasmid px458-253r or 10 μg E64. (C) Overexpression or injection with rCTSL: CTSL concentration increased when injected with 30 μg rCTSL, while only slightly increased with 5 μg rCTSL; CTSL was overexpressed when transinfected with eukaryotic plasmid CTSL-GFP from 0.5 μg to 2.0 μg. (D) Levels of SIgA increased significantly when DCs+B cells were treated with 30 μg rCTSL or 2.0 μg CTSL-GFP. Data represent means ± SEM from three independent experiments (**p<0.01, ***p<0.001).