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. 2019 Apr 15;14(4):e0215408. doi: 10.1371/journal.pone.0215408

Fig 3. Cytokines concentrations were measured in cell culture’s supernatants by ELISA.

Fig 3

(A) Concentration of TGF-β: DCs+B cells pretreated with rCTSL protein or CTSL-GFP eukaryotic plasmid had a significant increase, while the concentration decreased when treated with inhibitor E64 or px458-253r. B cells alone and DC cells alone had slight difference among different treatment. (B) Concentration of IFN-γ: when CTSL was overexpressed or increased in co-cultured cells, the concentration increased; by contrast, the concentration decreased when CTSL was inhibited or knockdown; single cells had a slight change. (C, D) Concentration of IL-6 and IL-10: neither IL-6 nor IL-10 showed significant differences among the three cell types. Data are representative of three independent experiments (*p<0.05, **p<0.01, ***p<0.001).