Fig 8. rCTSL enhanced immune responses.

(A) SIgA in BALFs: the level of SIgA significantly increased at 14 and 21 DPI when treated with rCTSL, and higher than M.hp group. Data represent means ± SEM from three independent experiments (**p<0.01, ***p<0.001). (B) MHC class II molecules were detected by IHC in lung samples: there was positive staining in lungs when pigs were infected with M.hp (arrow); reaction was stronger in rCTSL+M.hp group (arrow). (C) FACS analysis of CD4 and CD8: CD8⁺T cells abounded in rCTSL+M.hp group at 21 DPI, CD4⁺ T cells stayed along with infection especially at the late stage.