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. 2019 Apr 15;14(4):e0215312. doi: 10.1371/journal.pone.0215312

Fig 1. Cleaning, albumen removal and windowing of the eggs.

Fig 1

A) Upon delivery eggs were cleaned either with ethanol or water (n = 20 each). Viability of the embryos was checked at the indicated time points (EDD = embryonic development day). B) Removal of albumen using diaphanoscopy, windowing of the eggs and resealing for further incubation. C) Viability before (n = 50) and after (n = 78) implementation of the described optimization steps (** p<0.01).