Database	Time span	Search strategy
The Cochrane Hepato‐Biliary Group Controlled Trials Register	Date of search	(immunoassay* or immunofluoroscenc* or immunoflorescenc* or IIF* or ELISA* or western blot* or WB* or LIA* or (antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)) or (anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML))) AND ((biliary and (cirrhosis or cholangitis)) or PBC)
The Cochrane Hepato‐Biliary Group Diagnostic Test of Accuracy Studies Register	Date of search	(immunoassay* or immunofluoroscenc* or immunoflorescenc* or IIF* or ELISA* or western blot* or WB* or LIA* or (antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)) or (anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML))) AND ((biliary and (cirrhosis or cholangitis)) or PBC)
The Cochrane Library	Latest issue	#1 MeSH descriptor: [Serologic Tests] explode all trees #2 MeSH descriptor: [Immunoassay] explode all trees #3 (immunoassay* or immunofluoroscenc* or immunoflorescenc* or IIF* or ELISA* or western blot* or WB* or LIA*) #4 MeSH descriptor: [Antibodies, Antinuclear] explode all trees #5 (antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)) #6 (anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML)) #7 #1 or #2 or #3 or #4 or #5 or #6 #8 MeSH descriptor: [Liver Cirrhosis, Biliary] explode all trees #9 ((biliary and (cirrhosis or cholangitis)) or PBC) #10 #8 or #9 #11 #7 and #10
MEDLINE (OvidSP)	January 1946 to the date of search	1. exp Serologic Tests/ 2. exp Immunoassay/ 3. (immunoassay* or immunofluorescenc* or immunoflorescenc* or IIF* or ELISA* or western blot* or WB* or LIA*).mp. [mp=title, abstract, original title, name of substance word, subject heading word, keyword heading word, protocol supplementary concept word, rare disease supplementary concept word, unique identifier] 4. exp Antibodies, Antinuclear/ 5. (antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)).mp. [mp=title, abstract, original title, name of substance word, subject heading word, keyword heading word, protocol supplementary concept word, rare disease supplementary concept word, unique identifier] 6. (anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML)).mp. [mp=title, abstract, original title, name of substance word, subject heading word, keyword heading word, protocol supplementary concept word, rare disease supplementary concept word, unique identifier] 7. 1 or 2 or 3 or 4 or 5 or 6 8. exp Liver Cirrhosis, Biliary/ 9. ((biliary and (cirrhosis or cholangitis)) or PBC).mp. [mp=title, abstract, original title, name of substance word, subject heading word, keyword heading word, protocol supplementary concept word, rare disease supplementary concept word, unique identifier] 10. 8 or 9 11. 7 and 10
Embase (OvidSP)	January 1947 to the date of search	1. exp serology/ 2. exp immunoassay/ 3. exp Western blotting/ 4. (immunoassay* or immunofluorescenc* or immunoflorescenc*or IIF* or ELISA* or western blot* or WB* or LIA*).mp. [mp=title, abstract, heading word, drug trade name, original title, device manufacturer, drug manufacturer, device trade name, keyword] 5. exp mitochondrion antibody/ 6. exp antinuclear antibody/ 7. (antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)).mp. [mp=title, abstract, heading word, drug trade name, original title, device manufacturer, drug manufacturer, device trade name, keyword] 8. (anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML)).mp. [mp=title, abstract, heading word, drug trade name, original title, device manufacturer, drug manufacturer, device trade name, keyword] 9. 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 10. exp primary biliary cirrhosis/ 11. ((biliary and (cirrhosis or cholangitis)) or PBC).mp. [mp=title, abstract, heading word, drug trade name, original title, device manufacturer, drug manufacturer, device trade name, keyword] 12. 10 or 11 13. 9 and 12
Science Citation Index Expanded (ISI Web of Science)	January 1898 to the date of search	#6 #5 AND #4 #5 TS=((biliary and (cirrhosis or cholangitis)) or PBC) #4 #3 OR #2 OR #1 #3 TS=(anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML)) #2 TS=(antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)) #1 TS=(immunoassay* or immunofluoroscenc* or immunofloroscenc* or IIF* or ELISA* or western blot* or WB or LIA*)
BIOSIS (ISI Web of Science)	January 1969 to the date of search	#6 #5 AND #4 #5 TS=((biliary and (cirrhosis or cholangitis)) or PBC) #4 #3 OR #2 OR #1 #3 TS=(anti* and (M2 or sp100 or sp‐100 or sp140 or sp‐140 or gp210 or gp‐210 or EPO or promyelocytic leuk*mia or PML)) #2 TS=(antibod* and (antimitochondrial or antinuclear or eosinophil peroxidase)) #1 TS=(immunoassay* or immunofluorescenc* or immunoflorescenc* or IIF* or ELISA* or western blot* or WB or LIA*)
Medion (www.mediondatabase.nl/)	Date of search	We will conduct five separate searches of the abstract using the terms: primary biliary cirrhosis primary biliary cholangitis PBC serological tests liver biopsy
ARIF (www.birmingham.ac.uk/research/activity/mds/projects/HaPS/PHEB/ARIF/databases/index.aspx)	Date of search	(primary biliary cirrhosis) OR PBC OR (serological tests) OR (liver biopsy)
EU Clinical trials register (www.clinicaltrialsregister.eu/)	Date of search	We will conduct two separate searches using the terms: primary biliary cirrhosis primary biliary cholangitis
Clinicatrials.gov (clinicaltrials.gov)	Date of search	We will conduct two separate searches using the terms: primary biliary cirrhosis primary biliary cholangitis