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. Author manuscript; available in PMC: 2020 Mar 5.
Published in final edited form as: Circulation. 2019 Mar 5;139(10):1300–1319. doi: 10.1161/CIRCULATIONAHA.118.036323

Table 1.

List of Cys-containing peptides generated from tryptic digest of recombinant GPIbα that are detected by mass spectrometry.

Disulfide bond Cys position Peptide sequence*
4–17 4
17		 HPICEVSK
VASHLEVNCDK
VASHLEVNCDKR
209–248 248 AMTSNVASVQCDNSDK
AM(oxidation)TSNVASVQCDNSDK
AMTSNVASVQCDNSDKFPVYK
AM(oxidation)TSNVASVQCDNSDKFPVYK
211–264 264 GCPTLGDEGDTDLYDYYPEEDTEGDK
GCPTLGDEGDTDLYDYYPEEDTEGDKVR
N/A 65 LTQLNLDRCELTK
CELTK
*

Cys was labelled with 12C-IPA (133.05276) or 13C-IPA (139.07289).