Figure 6.

Akt signaling positively regulates sema3a and slit1 in the forebrain. Plasmids encoding DN AKT (A, B) ^T308A/S473APKB, and CA AKT (C, D) ^T308D/S473DPKB, and (E, F) AKT-myr were electroporated into the forebrain of stage 27/28 embryos. A, C, E, Forebrain transgene expression was assessed by immunohistochemistry for HA-tagged AKT mutants. B, D, F, spry1, sema3a, and slit1 expression in the forebrain 24 h after electroporation was quantified by RT-qPCR for (B) ^T308A/S473APKB (n = 21, N = 3 vs control n = 23, N = 3), (D) ^T308D/S473DPKB (n = 40, N = 5 vs control n = 39, N = 5), and (F) AKT-myr (n = 22, N = 3 vs control n = 24, N = 3), where the control electroporation was pCS2-GFP. Bars represent the mean ± SEM; *p < 0.05 using the REST algorithm for statistical significance. Scale bar, 50 µm. ve, ventricle.