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. 2019 Apr 9;6(2):ENEURO.0025-19.2019. doi: 10.1523/ENEURO.0025-19.2019

Figure 1.

Figure 1.

Axotomy-induced recombination in peripherally-projecting neurons. A, Validation of an ATF3-specific antibody. The Novus antibody (NBP1-85816) produces a positive signal in nuclei of axotomized sensory neurons in ATF3+/+ mice, but not ATF3cre/cre mice. Note that the Santa Cruz antibody (C-19) labels neuronal nuclei in in the latter (inset), indicating non-specific staining. B, C, Axotomy induced reporter expression in sensory (DRG), sympathetic (stellate ganglion, SG), and motoneurons 4 d after injury. D, Reporter expression in sensory axons and motoneurons one week after injury. E, Preventing CreERT2 translocation from cytoplasm to nucleus with ICI 182780 reduces recombination in ATF3+ cells (by ∼50%). F, Recombination efficiency 16 d after injury was calculated by expressing the proportion of tracer-filled somata (labeled at the time of injury) that were also reporter (tdtomato)-positive. G, Recombination efficiencies at 4 and 16 d after injury (n = 3 for each time point) for DRG and motoneurons. Images in panels A, B, E were taken from whole mounts, those in C, D, F from cryosections.