| Study characteristics |
| Patient sampling |
Consecutive enrollment of infants (24 were neonates) with signs of systemic inflammatory response syndrome and risk factors for candidemia. |
| Patient characteristics and setting |
Infants who were admitted to the ICU of 2 pediatric hospital in Sao Paulo State, Brazil over an 18‐month period. Study period (month and year) or participant demographics not available. Author provided results for the 24 neonates. |
| Index tests |
Multiplex nested PCR with specific primers designed to identify 7 Candida species |
| Target condition and reference standard(s) |
Candidemia and blood culture. |
| Flow and timing |
Blood sample for both culture and PCR were done concurrently. |
| Comparative |
|
| Notes |
Data based on email communication with Dr. Del Negro. |
| Methodological quality |
| Item |
Authors' judgement |
Risk of bias |
Applicability concerns |
| DOMAIN 1: Patient Selection |
| Was a consecutive or random sample of patients enrolled? |
Yes |
|
|
| Was a case‐control design avoided? |
Yes |
|
|
| Did the study avoid inappropriate exclusions? |
Yes |
|
|
| |
|
Low |
Unclear |
| DOMAIN 2: Index Test All tests |
| Were the index test results interpreted without knowledge of the results of the reference standard? |
Yes |
|
|
| If a threshold was used, was it pre‐specified? |
Yes |
|
|
| |
|
Low |
Low |
| DOMAIN 3: Reference Standard |
| Is the reference standards likely to correctly classify the target condition? |
Yes |
|
|
| Were the reference standard results interpreted without knowledge of the results of the index tests? |
Yes |
|
|
| |
|
Low |
Low |
| DOMAIN 4: Flow and Timing |
| Was there an appropriate interval between index test and reference standard? |
Yes |
|
|
| Did all patients receive the same reference standard? |
Yes |
|
|
| Were all patients included in the analysis? |
Yes |
|
|
| |
|
Low |
|
