Fig 3. Expression of the gap-junction proteins connexin 26, connexin 32, and connexin 43 in H69 cells after treatment with NDMA and/or ESP, as determined by western blotting.

H69 cells were incubated with either PBS (vehicle) or NDMA and/or ESP for 72 h, and the cells were collected for protein extraction. A. The blots of each groups were run under same experimental conditions and the images were cropped from different parts of the same gels. B-D. Quantification of relative Cx43 Cx26, and Cx32 expression in each group. The each proteins level are indicated as normalization of the ratio of Cx43/Calnexin, Cx26/Calnexin, and Cx32/Calnexin, and Cox-2/Calnexin. DATA represent the mean ± SE of five independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus control.