Table 2. Oligonucleotide primers and detection probe for real-time PCR.
Oligonucleotide name |
Oligonucleotide sequences |
---|---|
Connexin 26 | |
GJB2 F GJB2 R GJB2 probe |
CCC CTA AAG CCT CAA AAC AAA G GAA ACA AAT GCC GAT ATC CTC TG 56-FAM/CCT TAC ACC /ZEN/AAT AAC CCC TAA CAG CCT /3IABkFQ |
Connexin 32 | |
GJB1 F GJB1 R GJB1 probe |
GCA CAG ACA TGA GAC CAT AGG CAA ACC TGT CCA GTT CAT CCT 56-FAM/CCT ATC CCT /ZEN/GAG GCC ACC CAG /3IABkFQ |
Connexin 43 | |
GJA1 F GJA1 R GJA1 probe |
ACT TGG CGT GAC TTC ACT AC AGC AGT TGA GTA GGC TTG AAC 56-FAM/AGG CAA CAT /ZEN/GGG TGA CTG GAG C/3IABkFQ |
Cox-2 | |
PTGS2 F PTGS2 R PTGS2 probe |
ACT TGG CGT GAC TTC ACT AC AGC AGT TGA GTA GGC TTG AAC /56-FAM/AGG CAA CAT /ZEN/GGG TGA CTG GAG C/3IABkFQ/ |
GAPDH | |
GAPDH F GAPDH R GAPDH probe |
ACA TCG CTC AGA CAC CAT G TGT AGT TGA GGT CAA TGA AGG G 5HEX/AAG GTC GGA /ZEN/GTC AAC GGA TTT GGT C/3IABkFQ |