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. 2019 Apr 3;13(4):e0006843. doi: 10.1371/journal.pntd.0006843

Table 2. Oligonucleotide primers and detection probe for real-time PCR.

Oligonucleotide
name
Oligonucleotide sequences
Connexin 26
GJB2 F
GJB2 R
GJB2 probe
CCC CTA AAG CCT CAA AAC AAA G
GAA ACA AAT GCC GAT ATC CTC TG
56-FAM/CCT TAC ACC /ZEN/AAT AAC CCC TAA CAG CCT /3IABkFQ
Connexin 32
GJB1 F
GJB1 R
GJB1 probe
GCA CAG ACA TGA GAC CAT AGG
CAA ACC TGT CCA GTT CAT CCT
56-FAM/CCT ATC CCT /ZEN/GAG GCC ACC CAG /3IABkFQ
Connexin 43
GJA1 F
GJA1 R
GJA1 probe
ACT TGG CGT GAC TTC ACT AC
AGC AGT TGA GTA GGC TTG AAC
56-FAM/AGG CAA CAT /ZEN/GGG TGA CTG GAG C/3IABkFQ
Cox-2
PTGS2 F
PTGS2 R
PTGS2 probe
ACT TGG CGT GAC TTC ACT AC
AGC AGT TGA GTA GGC TTG AAC
/56-FAM/AGG CAA CAT /ZEN/GGG TGA CTG GAG C/3IABkFQ/
GAPDH
GAPDH F
GAPDH R
GAPDH probe
ACA TCG CTC AGA CAC CAT G
TGT AGT TGA GGT CAA TGA AGG G
5HEX/AAG GTC GGA /ZEN/GTC AAC GGA TTT GGT C/3IABkFQ