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. 2019 Mar 28;18(6-7):759–770. doi: 10.1080/15384101.2019.1593708

Figure 4.

Figure 4.

RPL22/eL22 directly binds and inhibits CDK4-cyclin D1 complex. (a) Immunoblots of the indicated proteins after in vitro kinase assay containing ATP and GST-CDK4 and GST-cyclin D1, with or without GST-RB (773–928), palbociclib and HIS-RPL22. RB(pS795): RB phosphorylated on serine 795. (b) In vitro GST pull-down of recombinant GST, GST-cyclin D1 and/or GST-CDK4 and recombinant HIS-RPL22 using glutathione beads. Lysate and pull-down were immunoblotted for the indicated proteins. (c) Model showing interactions between RPL22 and cyclin D1, CDK4 or CDK4-cyclin D1. (d) Model showing how nucleolar stress can lead to activation of p53 and RB tumor suppressor pathways. Blots in A and B are representative of 3 independent experiments with similar results.