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. 2019 Mar 28;18(6-7):759–770. doi: 10.1080/15384101.2019.1593708

Figure 3.

Figure 3.

E6 or E7 can bypass RPL22/eL22-induced senescence. (a) Growth curves of IMR90 cells expressing RPL22-Myc or an empty control vector (Vect) in combination with the expression of the viral oncoproteins E6, E7 or an empty control vector (Vect). Data are presented as means normalized to day 0 of each condition and error bars indicate SEM, n = 3 independent experiments. (b) SA-β-gal for cells as in (a) at day 12 post-infection. Data were quantified from 5-independent cell counts up to a total of at least 150 cells in triplicate and are presented as the mean percentage of positive cells ± SD. Scale bar, 200 µm. (c) Immunoblots of indicated proteins performed on cells as in (a) at day 12 post-infection. RB(tot): total RB; H3(pS10): histone H3 phosphorylated on serine 10; p53(tot): total p53; RPL22(endo): endogenous RPL22. (d) Immunoblots of indicated proteins performed on IMR90 cells expressing RPL22-Myc or an empty control vector (Vect) in combination with the expression of a control shRNA (shNTC), a shRNA against p53 (shp53) or a shRNA against RB (shRB) at day 12 post-infection. RB(tot): total RB; H3(pS10): histone H3 phosphorylated on serine 10; p53(tot): total p53; RPL22(endo): endogenous RPL22. Blots in C and D are representative of 3 independent experiments with similar results.