Figure 8. Neonatal inflammation does not alter GFAP or IBA1 immunofluorescence in adult preBötzinger Complex or ventral cervical spinal cords.

After neonatal LPS (1 mg/kg, i.p., (P4), representative confocal images (40x) from adult preBötC (A and B) and cervical spinal cords (C and D) displayed no qualitative differences in immunoreactivity for GFAP (green, astrocytes) or IBA1 (green, microglia) in males (left panels) or females (right panels). PreBötC neurons are labeled with antibodies for NK1R (red, A and B) and motor neurons are labeled with antibodies for ChAT (red, C and D). Neonatal inflammation did not significantly change mean fluorescent intensity of either GFAP or IBA1 in the preBötC (E) or cervical spinal cord (F), suggesting no lasting differences in astrocytes or microglia after neonatal inflammation. Scale bars: 50 µm.