Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | LPS (Lipopolysaccharides from e Coli (0111:B4)) | Sigma Aldrich | L4130 | dissolved in saline, 1 mg/ml |
| Chemical compound, drug | Keto ((S) - (+) - Ketoprofen) | Sigma Aldrich | 471909 | dissolved in 50% ethanol in saline,12.5 mg/ml |
| Chemical compound, drug | CGS-21680 | Sigma Aldrich | C141 | dissolved in DMSO to50 mM for storage in aliquots. Dissolved to 100 uM in 10% DMSO and artificial CSF for injections. |
| Antibody | anti-GFAP (Rabbit polyclonal) | Millipore | (Millipore Cat# AB5804, RRID:AB_2109645) | (1:1000) |
| Antibody | anti-NK1R (Guinea pig polyclonal) |
Millipore | (Millipore Cat# AB15810, RRID:AB_11213393) |
(1:500) |
| Antibody | anti-IBA1 (Rabbit polyclonal) | Wako | (Wako Cat# 019–19741, RRID:AB_839504) | (1:1000) |
| Antibody | anti-CHaT (Goat polyclonal) | Millipore | (Millipore Cat# AB144P, RRID:AB_2079751) | (1:300) |
| Antibody | donkey-anti-rabbit 647 IgG secondary | Life Technologies | (Molecular Probes Cat# A-31573, RRID:AB_2536183) | (1:1000) |
| Antibody | donkey-anti-goat 555 IgG secondary | Life Technologies | (Molecular Probes Cat# A-21432, RRID:AB_141788) | (1:1000) |
| Antibody | donkey-anti-guinea pig 488 IgG secondary | Jackson Immuno | (Jackson ImmunoResearch Labs Cat# 706-545-148, RRID:AB_2340472) | (1:1000) |
| Sequence-based reagent | IL-1β forward primer | Integrated DNA Technologies | CTG CAG ATG CAA TGG AAA GA | |
| Sequence-based reagent | IL-1β reverse primer | Integrated DNA Technologies | TTG CTT CCA AGG CAG ACT TT | |
| Sequence-based reagent | IL-6 forward primer | Integrated DNA Technologies | GTG GCT AAG GAC CAA GAC CA | |
| Sequence-based reagent | IL-6 reverse primer | Integrated DNA Technologies | GGT TTG CCG AGT AGA CCT CA | |
| Sequence-based reagent | iNOS forward primer | Integrated DNA Technologies | AGG GAG TGT TGT TCC AGG TG | |
| Sequence-based reagent | iNOS reverse primer | Integrated DNA Technologies | TCT GCA GGA TGT CTT GAA CG | |
| Sequence-based reagent | TNFα forward primer | Integrated DNA Technologies | TCC ATG GCC CAG ACC CTC ACA C | |
| Sequence-based reagent | TNFα reverse primer | Integrated DNA Technologies | TCC GCT TGG TGG TTT GCT ACG | |
| Sequence-based reagent | COX2 forward primer | Integrated DNA Technologies | TGT TCC AAC CCA TGT CAA AA | |
| Sequence-based reagent | COX2 reverse primer | Integrated DNA Technologies | CGT AGA ATC CAG TCC GGG TA | |
| Sequence-based reagent | 18 s forward primer | Integrated DNA Technologies | CGG GTG CTC TTA GCT GAG TGT CCC | |
| Sequence-based reagent | 18 s reverse primer | Integrated DNA Technologies | CTC GGG CCT GCT TTG AAC AC |