Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional Information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
Bcl6fl/fl | PMID 30566857 | ||
| Genetic reagent (Mus musculus) |
Albumin-cre | Jackson Laboratory | Stock #003574 | |
| Genetic reagent (Mus musculus) |
Ppara-/- | Jackson Laboratory | Stock #008154 | |
| Genetic reagent (Mus musculus) |
Ppardfl/fl | Jackson Laboratory | Stock #005897 | |
| Antibody | anti-BCL6 (guinea pig polyclonal) |
PMID 30566857 | custom polyclonal 7.5 μg per IP |
|
| Antibody | anti-PPARδ (guinea pig polyclonal) |
PMID 28467934 | custom polyclonal 7.5 μg per IP |
|
| Antibody | anti-SMRT (guinea pig polyclonal) |
PMID 22465074 | custom polyclonal 7.5 μg per IP |
|
| Antibody | anti-NCoR (guinea pig polyclonal) |
PMID 22465074 | custom polyclonal 7.5 μg per IP |
|
| Antibody | anti-HDAC3 (rabbit polyclonal) |
Santa Cruz | Cat. #: sc-11417x | 5 μg per IP |
| Antibody | anti-H3K27ac (rabbit polyclonal) |
Active Motif | Cat. #: 39133 | 5 μg per IP |
| Antibody | anti-pAKT (rabbit monoclonal) |
Cell Signaling | Cat. #: 4060 s | (1:1000) |
| Antibody | anti-panAKT (rabbit monoclonal) |
Cell Signaling | Cat. #: 4691 s | (1:1000) |
| Antibody | anti-BCL6 (mouse monoclonal) |
Santa Cruz | Cat. #: sc7388 | (1:200) |
| Antibody | anti-β-Actin (mouse monoclonal) |
Sigma-Aldrich | Cat. #: A1978 | 1:1000) |
| Antibody | anti-PPARα (rabbit polyclonal) |
Santa Cruz | Cat. #: sc-9000x | 7.5 μg per IP; WB: (1:500) |
| Antibody | Peroxidase AffiniPure Goat Anti-Mouse IgG |
Jackson ImmunoResearch | Cat. #: 115-035-174 | (1:20,000) |
| Antibody | Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG |
Jackson ImmunoResearch | Cat. #: 211-032-171 | (1:20,000) |
| Antibody | Rabit Anti-guinea pig IgG H and L |
Abcam | Cat. #: ab6698 | |
| Chemical compound, drug | DSG Crosslinker | ProteoChem | Cat. #: c1104 | |
| Chemical compound, drug | Formaldehyde, 16%, methanol-free, Ultra Pure |
Polysciences, Inc | Cat. #: 18814–20 | |
| Chemical compound, drug | RNAlater Stabilization Solution | ThermoFisher Scientific | Cat. #: AM7020 | |
| Chemical compound, drug | TRIzol Reagent | ThermoFisher Scientific | Cat. #: 15596018 | |
| Chemical compound, drug | Poloxamer 407 | Sigma-Aldrich | Cat. #: 16758 | |
| Chemical compound, drug | cOmplete Ultra Tablets, EDTA-free |
Sigma-Aldrich | Cat. #: 5892953001 | |
| Chemical compound, drug | Deuterium oxide | Sigma-Aldrich | Cat. #: 151882 | |
| Chemical compound, drug | Sodium Palmitate | Sigma-Aldrich | Cat. #: P9767 | |
| Chemical compound, drug | Palmitic Acid, [1–14C] | MP Biomedicals | Cat. #: 12195 | |
| Chemical compound, drug | BODIPY 500/510 C1, C12 | Invitrogen | Cat. #: D3823 | |
| Chemical compound, drug | Humulin R | Lilly | NDC 0002-8215-01 | |
| Commercial assay or kit | Dynabeads M-280 Sheep Anti-Rabbit IgG |
Invitrogen | Cat. #: 11204D | |
| Commercial assay or kit | Dynabeads M-280 Tosylactivated |
Invitrogen | Cat. #: 14204 | |
| Commercial assay or kit | Protein A Agarose/Salmon Sperm DNA |
Millipore | Cat. #: 16–157 | |
| Commercial assay or kit | iScript cDNA Synthesis Kit | BioRad | Cat. #: 1708891 | |
| Commercial assay or kit | iTaq Universal SYBR Green | BioRad | Cat. #: 1725124 | |
| Commercial assay or kit | Infinity Triglyceride Assay Kit | ThermoFisher Scientific | Cat. #: TR22421 | |
| Commercial assay or kit | β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit |
Cayman Chemical | Cat. #: 700190 | |
| Commercial assay or kit | Ultra Sensitive Mouse Insulin ELISA Kit |
Crystal Chem | Cat. #: 90080 | |
| Commercial assay or kit | Glucose Colorimetric /Fluorometric Assay Kit |
BioVision | Cat. #: K606 | |
| Commercial assay or kit | Microvette CB 300 K2E | Sarstedt | Cat. #: 16.444 | |
| Commercial assay or kit | MemCode Reversible Protein Stain Kit |
ThermoFisher Scientific | Cat. #: 24585 | |
| Commercial assay or kit | Whatman qualitative filter paper | Sigma-Aldrich | Cat. #: WHA1003055 | |
| Commercial assay or kit | KAPA Hyper Prep Library Prep Kit | Kapa Biosystems | Cat. #: KK8504 | |
| Commercial assay or kit | KAPA Stranded RNA-seq Kit with RiboErase |
Kapa Biosystems | Cat. #: KK8483 | |
| Commercial assay or kit | NextSeq 500/550 High Output Kit v2.5 (75 cycles) |
Illumina | Cat. #: 20024906 | |
| Other, research diet (45% kcal from fat) |
HFD | Research Diets, Inc | Stock #D12451 |