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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2016 Feb 17;196(6):2788–2798. doi: 10.4049/jimmunol.1502106

Fig. 1. Polymicrobial sepsis leads to host and bacterial RNA release into the blood circulation.

Fig. 1

Twenty-four hours after sham or CLP surgery, mice were euthanized and blood was collected via inferior vena cava. RNA was extracted from plasma and quantified as described in Methods. A. Plasma total RNA concentrations in sham and CLP mice. * P < 0.05. n=8 in sham group, n=10 in CLP group. B-C. Association of plasma RNA concentration and sepsis severity score or core temperature, respectively. Sepsis severity score was monitored as detailed in the Methods. Rectal temperature was recorded as the core temperature. n=18 pair samples. D. Bacterial 16S rRNA in the plasma as measured by qRT-PCR. E. Host GAPDH mRNA in the plasma as measured by qRT-PCR, * P < 0.05. n=8 in sham group, n=10 in CLP group.