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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2016 Feb 17;196(6):2788–2798. doi: 10.4049/jimmunol.1502106

Fig. 9. Cellular RNA and miR-146a mimics stimulate cfB production in vivo via TLRs signaling.

Fig. 9

RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with lipofectamine was injected i.p. into WT, MyD88−/− or TLR7−/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P < 0.05, **** P < 0.0001. B. cfB expression in the peritoneal lavage after miRNA-146a injection. n=2–3 per group, **** P < 0.0001. None, no injection; Mutant, miR-146a-mutant.