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(A) Mass spectrum and (B) extracted ATD of [M + 14H+]14+ apo-streptavidin and the holo-streptavidin complexes. A small shift in drift time was observed upon binding four biotin molecules as well as a compaction of peak width. *Denotes additional N-terminal methionine residues on streptavidin and were not included in the extracted ATDs.
