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. 2019 Apr 2;8:e39159. doi: 10.7554/eLife.39159

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© 2019, Celaya et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 6. — (A) Cochlear expression of inflammatory response genes in Dusp1^+/+ (lighter color bars) and Dusp1^–/– mice (darker color bars) of 2, 4–5 and 8–9 months of age. Expression levels were calculated as 2^–ΔΔCt (RQ), using Rplp0 as the reference gene and normalized to the 2-month-old wildtype mice group. Il10 data were normalized to the matched-age wildtype mice groups. Values are presented as mean ± SEM of triplicates from pool samples of three mice per condition. (B) Representative microphotographs of cochlear mid-modiolar cryosections immunolabeled for IBA1, showing a detail of the spiral ligament of the apical, middle and basal turns of 5-month-old Dusp1^+/+ (n = 3) and Dusp1^–/– mice of 5 (n = 3) and 8 months of age (n = 3). The Type IV fibrocytes region is outlined. Arrowheads point to macrophage cells. Scale: 50 µm. (C) IBA1 total fluorescence intensity was measured in the spiral ligament in each cochlear turn of 5-month-old Dusp1^+/+ (light green bars; base, n = 4; middle, n = 5; apical, n = 4) and Dusp1^–/– mice of 5 months (dark green bars, base, n = 4; middle, n = 6; apical, n = 5) and 8 months of age (dark blue bars, base, n = 4; middle, n = 3; apical, n = 3). Values are presented as mean ± SEM. Statistically significant differences were detected by Student’s t-test comparing genotypes (*p<0.05, **p<0.01, ***p<0.001).

Figure 6—figure supplement 1. — (A) Cochlear expression of inflammatory response genes in Dusp1^+/+ (lighter color bars) and Dusp1^–/– mice (darker color bars) of 2, 4–5 and 8–9 months of age. Expression levels were calculated as 2^–ΔΔCt (RQ), using Rplp0 as the reference gene and normalized to the 2-month-old wildtype mice group. Il10 data were normalized to the matched-age wildtype mice groups. Values are presented as mean ± SEM of triplicates from pool samples of three mice per condition. (B) Representative microphotographs of cochlear mid-modiolar cryosections immunolabeled for IBA1, showing a detail of the spiral ligament of the apical, middle and basal turns of 5-month-old Dusp1^+/+ (n = 3) and Dusp1^–/– mice of 5 (n = 3) and 8 months of age (n = 3). The Type IV fibrocytes region is outlined. Arrowheads point to macrophage cells. Scale: 50 µm. (C) IBA1 total fluorescence intensity was measured in the spiral ligament in each cochlear turn of 5-month-old Dusp1^+/+ (light green bars; base, n = 4; middle, n = 5; apical, n = 4) and Dusp1^–/– mice of 5 months (dark green bars, base, n = 4; middle, n = 6; apical, n = 5) and 8 months of age (dark blue bars, base, n = 4; middle, n = 3; apical, n = 3). Values are presented as mean ± SEM. Statistically significant differences were detected by Student’s t-test comparing genotypes (*p<0.05, **p<0.01, ***p<0.001).