Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
Dusp1-/- | Dorfman et al., 1996 | RRID:MGI:4940296 | R Perona lab (Institute of Biomedical Research 'Alberto Sols', Madrid, Spain) |
| Genetic reagent (M. musculus) |
Mapk14+/KI; Mapk14KI/KI | Ventura et al., 2007 | RRID:MGI:3716853 | AR Nebreda lab (Institute for Research in Biomedicine, Barcelona, Spain) |
| Cell line (M. musculus) |
Dusp1–/– MEF | Dorfman et al., 1996 | RRID:MGI:4940296 | Primary culture of mouse embryonic fibroblasts, maintained in I Sánchez-Pérez's lab |
| Antibody | Anti-myelin P0 (chicken polyclonal) |
Neuromics | Neuromics Cat# CH23009; RRID:AB_2737144 |
IHC (1:150) |
| Antibody | Anti-nitrotyrosine (rabbit polyclonal) |
Merck-Millipore | Millipore Cat# AB5411; RRID:AB_177459 |
IHC (1:100) |
| Antibody | Anti-IBA1 (goat polyclonal) | Abcam | Abcam Cat# ab5076; RRID:AB_2224402 |
IHC (1:100) |
| Antibody | Anti-MyoVIIA (rabbit polyclonal) |
Proteus | Proteus Biosciences Cat# 25–6790; RRID:AB_2314838 |
IHC (1:250) |
| Antibody | Anti-neurofilament (mouse monoclonal) |
Merck-Millipore | Millipore Cat# CBL212; RRID:AB_93408 |
IHC (1:100) |
| Antibody | Alexa Fluor 647 Phalloidin | Thermo Fisher Scientific | Thermo Fisher Scientific Cat# A22287; RRID:AB_2620155 |
IHC (1:1000) |
| Antibody | Anti-P-p38 (rabbit polyclonal) |
Cell Signaling | Cell Signaling Technology Cat# 9211, RRID:AB_331641 |
WB (1:1000; 1:2000) |
| Antibody | Anti-P-JNK (rabbit polyclonal) |
Cell Signaling | Cell Signaling Technology Cat# 4668; RRID:AB_823588 |
WB (1:1000) |
| Antibody | Anti-P-ERK (rabbit polyclonal) |
Cell Signaling | Cell Signaling Technology Cat# 9101, RRID:AB_331646 |
WB (1:1000) |
| Antibody | Anti-MKP1 (C-19) (rabbit polyclonal) |
Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-370; RRID:AB_631385 |
WB (1:1000) |
| Antibody | Anti-γ-H2AX Ser139 (rabbit polyclonal) |
Cell Signaling | Cell Signaling Technology Cat# 2577; RRID:AB_2118010 |
IHC (1:200); WB (1:1000) |
| Antibody | Anti-P22phox (rabbit polyclonal) |
Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-20781; RRID:AB_2090309 |
WB (1:250) |
| Antibody | Anti-MnSOD (rabbit polyclonal) |
Merck-Millipore | Millipore Cat# 06–984; RRID:AB_310325 |
WB (1:1000) |
| Antibody | Anti-BAX (NT) (rabbit polyclonal) |
Merck-Millipore | Millipore Cat# ABC11; RRID:AB_10561771 |
WB (1:1000) |
| Antibody | Anti-PI3K (rabbit polyclonal) |
Not commercially available |
WB (1:10,000) From AM Valverde's lab (Institute of Biomedical Research 'Alberto Sols', Madrid, Spain) |
|
| Antibody | Anti-p38 (C-20) (rabbit polyclonal) |
Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-535; RRID:AB_632138 |
WB (1:1000) |
| Antibody | Anti-caspasa3 (rabbit polyclonal) |
Cell Signaling | Cell Signaling Technology Cat# 9662; RRID:AB_331439 |
WB (1:1000) |
| Antibody | Anti-β-actin (mouse monoclonal) |
Sigma-Aldrich | Sigma-Aldrich Cat# A5441; RRID:AB_4767441 |
WB (1:10000) |
| Antibody | Anti-β-tubulin (mouse monoclonal) |
Sigma-Aldrich | Sigma-Aldrich Cat# T9026; RRID:AB_47759 |
WB (1:1000) |
| Commercial assay or kit |
Dead-End Fluorometric TUNEL System |
Promega | Promega Cat# G3250 |
|
| Chemical compound, drug |
TNFα | Sigma-Aldrich | Sigma-Aldrich Cat# T6674 |
(10 ng/ml) |
| Software, algorithm | BioSigRP TM | Tucker Davis Technologies (TDT) |
RRID:SCR_014590 | |
| Software, algorithm | Fiji | Fiji (https://fiji.sc/) | RRID:SCR_002285 | |
| Software, algorithm | SPSS | IBM | RRID:SCR_002865 | |
| Software, algorithm | Cell Profiler | Cell Profiler (https://cellprofiler.org/) |
RRID:SCR_007358 |