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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Neurogenet. 2018 Sep 11;32(4):336–352. doi: 10.1080/01677063.2018.1493479

Figure 4 – Jaw muscle fibers and branchiomotor innervation are intact in dscamb mutants.

Figure 4 –

A) Ventral view of head in 4dpf larvae with Et1(dscambt2b:Gal4) expression in jaw muscle fibers in heterozygous (A1) and homozygous (A2) mutants. Arrowheads: BMN axons, dotted lines: jaw muscle fibers (MFs). Scale bar: 100μm

B, C) Dorsal images of hindbrain regions in 4dpf larvae with Et1(dscambt2b:Gal4) in red and BMNs in green [Tg(isl1:GFP)]. Confocal projections of the facial (B) and vagal (C) BMN nuclei. Arrowheads: examples of mCherry+/GFP+ BMNs. Scale bars: 50μm

D) Confocal projections of ventral head in 7dpf heterozygous (D1, E1) and homozygous (D2, E2) mutants with NMJs labeled presynaptically (SV2, red) and postsynaptically (aBTX, green). E1, E2 are magnifications of dashed boxes in D1, D2, showing NMJs on the INTM-A and INTMP muscles. Scale bar D1: 100μm, scale bar E1: 50μm

F, G) Quantification of aBTX-stained object number (F) and median volume on the INTM-A and INTM-P of 7dpf heterozygous and homozygous mutant larvae. N = 16 hets and 13 muts. Mann-Whitney-Wilcoxon test: p = 0.98 for F and p = 0.46 for G.

Middle line is the median; lower and upper ends of boxes are 25th and 75th percentiles.