Figure 4.

The comparison of the amino acid residues in the ATP-binding and DFG motif-1 sites between anaplastic lymphoma kinase (ALK) and the insulin receptor (INSR). The similarities in the amino acid sequences around the ATP-binding site and DFG motif-1 between ALK and INSR are shown. Matched amino acid residues are highlighted in yellow. The gatekeeper and DFG motif-1 of ALK are L1196 and G1269 (indicated with red), respectively. Ceritinib binds to ¹¹⁹⁸LMA¹²⁰⁰, known as the triad hinge of ALK as well as a key site for ATP binding (indicated by a blue box). The DFG motif-1 and LMA triad hinge are highly conserved between ALK and the INSR, aside from the difference in the gatekeeper (L1196 for ALK and M1103 for INSR).