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. Author manuscript; available in PMC: 2020 Jan 4.
Published in final edited form as: J Proteome Res. 2018 Dec 12;18(1):426–435. doi: 10.1021/acs.jproteome.8b00699

Figure 2.

Figure 2.

DIA-based proteomic analysis in tumor biopsies. (A) Detected 4,239 proteins (DIA library) were categorized based on their sub-cellular localization. (B) Binned protein identifications from the chromatogram library (Library) generated using gas phase fractionation and from individual single-shot analysis of samples (SS-DIA) are compared with findings of Wisniewski et al.30–31. (C) Comparison findings [reference 32] are listed by decreasing copies per cell; 95% of our single-shot protein identifications are found between 5 × 106 and 2.5 × 103 copies per cell (n=3,573 matched protein identifications). Eight examples of proteins identified in single-shot analysis spanning the range of copies per cell were indicated. (D) Oncologic biomarkers matched to those cataloged in the Cancer Genome Interpreter database 32. 40 biomarkers at the protein level were detected and quantified across 12 samples.