Table 4.

N-Glycosylation Gain or Lost Due to Variant

protein name	peptide	variant	N-X-S/T/C	gain or lost	detected in sample
GC-rich sequence DNA-binding factor 2	DIDLSCGSGSSK	N211S	yes	lost	9 cells
NSFL1 cofactor p47	ASSSILINESEPTTNIQ1R	D290N	yes	gain	9 cells, 5000 cells
Keratin	VIDDTDITR	N193D	yes	lost	9 cells, 5000 cells
MKI67 FHA domain-interacting nucleolar phosphoprotein	YENESLQSGR	N104S	yes	lost	5000 cells
GC-rich sequence DNA-binding factor 2	DIDLSCGSGSSK	N211S	yes	lost	5000 cells
Mab-21 domain containing 1	GAPMDPNESPAAPEAALPK	T35N	yes	gain	5000 cells
Polyribonucleotide nucleotidyltransferase 1	EILQIMDK	N590D	yes	lost	5000 cells
annexin A1	GGPGSAVSPYPTFDPSSDVAALHK	N43D	yes	lost	5000 cells
Inner membrane protein, mitochondrial	QKGDTPASATAPTEAAQNISAAGDTLSVPAPAVQPEESLK	I148N	yes	gain	5000 cells