Figure 7:

Photomicrographs of neuronal nitric oxide synthase (nNOS) immunostaining in the rostral portion of the frontal lobe of the lar gibbon showing the distribution of WMICs immunoreactive to nNOS. (a) Moderately magnified image of the superior frontal gyrus (from the region indicated by the b in Fig. 1a), showing the numerous nNOS-immunoreactive WMICs deep to the cerebral cortex (grey matter, GM, white matter, WM). The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (b) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the superior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex. Arrows in a and b indicate the same neuron for orientation of image location. (c) Moderately magnified image of the fundus of the inferior frontal gyrus (from the region indicated by the d in Fig. 1a), showing the nNOS-immunoreactive WMICs deep to the cerebral cortex (GM) within the WM. The approximate boundary of the deep border of cortical layer VI and the WM is marked by a dashed line. (d) High magnification image of the cortical/white matter boundary (marked by a dashed line) of the fundus of the inferior frontal gyrus, showing the nNOS-immunoreactive WMICs deep to the cerebral cortex within the WM. Arrows in c and d indicate the same neuron for orientation of image location. Scale bar in c = 500 μm and apply to a and c. Scale bar in d = 250 μm and applies to b and d. In all images dorsal is to the top of the image and medial to the left.