. Author manuscript; available in PMC: 2020 Jul 1.

Published in final edited form as: J Comp Neurol. 2018 Oct 30;527(10):1633–1653. doi: 10.1002/cne.24545

Table 2:

Stereological parameters used for estimating neuronal numbers in the white matter of the lar gibbon. NeuN – neuronal nuclear marker; nNos – neuronal nitric oxide synthase; CR – calretinin.

Stain	Sectioning Plane	Counting frame size (μm)	Sampling grid size (μm)	Disector height (μm)	Section cut thickness (μm)	Measured mounted thickness (μm)	Upper and lower guard zones (μm)	Section interval	Number of sections	Number of sampling sites	Number of objects counted
NeuN	Coronal	200 × 200	1500 × 1500	19	50	26.5	2	120	10	662	2843
nNOS	Coronal	200 × 200	1500 × 1500	20	50	25.4	2	120	10	763	299
CR	Coronal	200 × 200	1500 × 1500	20	50	25.9	2	120	10	820	403