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. Author manuscript; available in PMC: 2020 Jan 4.
Published in final edited form as: J Proteome Res. 2018 Dec 10;18(1):309–318. doi: 10.1021/acs.jproteome.8b00623

Figure 4.

Figure 4

Ribosome proteomics reveals K63 ubiquitin-dependent binding of translation factors. (A) Scheme of polysome proteomics method following sucrose sedimentation profiling. (B) Scatter plot of log2 SILAC WT/K63R ratios of protein intensity from two independent replicates in the monosome fraction. (top) SILAC ratio of translation factors, (bottom) SILAC ratio of ribosomal proteins. Subunits of the eIF3 translation initiation factor are labeled in teal. WT, wild-type GMS280 yeast strain. K63R, ubiquitin K63R mutant GMS413 yeast strain.