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. Author manuscript; available in PMC: 2020 Jan 4.
Published in final edited form as: J Proteome Res. 2018 Dec 10;18(1):309–318. doi: 10.1021/acs.jproteome.8b00623

Figure 5.

Figure 5

Tif35 and eEF2 are depleted in K63R ubiquitin polysomes. (A,B) Sucrose sedimentation profiles of polysomes from wild-type and K63R mutant strain expressing HA-tagged Tif35 (A) untreated or (B) subjected to 0.6 mM H2O2 for 30 min. (C,D) Sucrose sedimentation profiles of polysomes from wild-type and K63R mutant strain expressing HA-tagged eEF2 (EFT1) (C) untreated or (D) subjected to 0.6mM H2O2 for 30 min. Western blot of sedimentation fractions were performed for anti-HA, 60S large protein Rpl10, and 40S small protein Rps6.