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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Plant J. 2019 Feb 12;98(2):359–369. doi: 10.1111/tpj.14210

Figure 3. Dual-target sRNA-FISH for maize anthers.

Figure 3.

(a) sRNA-FISH detected both zma-miR2275 (detected in the AF633 channel; magenta) and the 24 nt phasiRNA (detected in the AF568 channel; cyan) in the tapetal layer and archesporial cells. Each image was collected in spectra mode with laser scanning confocal microscopy and then spectrally unmixed using Zen Software. Bright-field and merged images were also shown for each image. TA, tapetal layer; AR, archesporial cells. Scale bars = 20 μm for all images. (b) Quantification of the AF633 and AF568 signal intensity in dual-target sRNA FISH and controls. (Significance level: < 0.05, *; < 0.01, **).