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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Plant J. 2019 Feb 12;98(2):359–369. doi: 10.1111/tpj.14210

Table 1:

Information on probes used in this study

miRNA miRNA abundance (TP10M) Probe sequence Probe Tm (°C) Hybridization temperature (°C) Probe concentration
zma-miR2118 4,064 /5DigN/TAGGAATGGGAGGCATCGGGAA 86 53.3 10 nM
24-phasiRNA 16,318 GGCCAAGGTCCGGTCCAACAACT/36-FAM/ 87 53.3 10 nM
zma-miR2275 12,100 GGTTCAGTTGCCTTAATCCT/3DigN/ 88 53.3 250 nM
24-phasiRNA for litchi 837 TCTCATGTTTTCTCATAGGTCT/3DigN/ 83 53.3 10 nM
Osa-miR1874 59,000 ATCGGGTTACACCTCCATCCATA/3Dig_N/ 86 53.3 100 nM
Scrambled control NA /5DigN/GTGTAACACGTCTATACGCCCA 87 53.3 10 nM

Footnote: Tm, melting temperature; 5DigN, 5’ Digoxigenin NHS Ester