Western blots demonstrate how differences in copper delivery route affect copper storage and trafficking protein expression. SDS/PAGE analysis of liver extracts from mice (n = 2–3) sacrificed either 6 or 24 hours after treatment with vehicle, Cu(gtsm), or Gal-Cu(gtsm) (0.75 mg Cu/kg mouse). Tissues were probed for ATP7B, CTR1, and MT-I/II. For CTR1, the monomer shown is for the unglycosylated region (~25 kDa).