(A) Cells cultured without added Mn, (B) cells exposed to 250 μM Mn, and (C) cells exposed to 500 μM Mn. From left to right panels, K and Mn distributions in cells displayed with a color scale from minimum (blue) to maximum (red) SXRF counts, overlay images of K (red) and Mn (green), and fluorescence microscopy overlay images of nucleus staining (blue) and Golgi apparatus labeling (red). Arrows indicate Mn and Golgi apparatus co-localization.