Under various performance metrics, read clouds (gold) consistently display superior performance in their ability to produce many complete and high-quality genome drafts as compared to either SLRs (blue) or short reads (green) approaches. Performance was also superior even in low short read coverage regimes (defined as <50x coverage). Counts include all complete/high-quality genome bins for all taxa in each approach.
a) Number of complete genome bins (>90% completeness, <5% contamination) with a minimum N50.
b) Number of complete genome bins with a minimum short read coverage depth. Genome bins with lower short read coverage correspond to less abundant organisms.
c) Number of complete genome bins with an N50 of >200kb and a minimum short read coverage depth.
d) Number of high-quality genome bins (complete and with at least 18 tRNAs, as well as at least one instance each of the 5S, 16S, and 23S rRNA genes) with a minimum N50.
e) Number of high-quality genome bins with a minimum short read coverage depth.
f) Number of high-quality genome bins with an N50 of >200kb and a minimum short read coverage depth.