Figure 4. cIAP1 sensitizes GBC cells to Caspase-8-associated apoptosis.

(A,B) LV-shcIAP1 group have Caspase-8 cleaved and activated more readily and severely than LV-NC in periodical Western blot in 6 h. (C,D) Another arrangement of group of various combinations of stimulation were treated with two GBC cells with indicated dose and time. The group treating TNF-α/cycloheximide showed obvious decrease in cIAP1 and increase in cleaved PARP, cleaved Caspase-3, and cleaved Caspase-8. Proteins with proximate molecular weight were performed by separated gels in the same electrophoresis with loading control of GAPDH in triplicate.