(A) GAS5 expression was determined by qRT-PCR in 210 paired CRC samples and adjacent normal tissues. (B,C) Analysis of GAS5 expression in CRC in TCGA database. (B) Expression of GAS5 in COAD (COAD, Colon adenoma; Normal n=41, Primary tumor n=286, ***P<0.001). (C) Expression of GAS5 in READ (READ, Rectal adenoma; Normal n=10, Primary tumor n=166, *P<0.05). (D) The expression levels of GAS5 were determined by qPCR in human normal colorectal mucosa cell NCM460 and CRC cell lines (**P<0.01). (E) GAS5 mRNA levels in CRC tissues expressing CT/TT or CC genotype were detected by qPCR analysis (*P<0.05). CT/TT, n=164, CC, n=46.