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. 2019 Apr 15;9:6107. doi: 10.1038/s41598-019-42554-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA (A) and OPLS-DA (B) scatter plots obtained from the matrix of metabolites for the 10 samples from siLUC (blue circles) and the 10 samples from siOPA1 (green circles). (A) PCA shows neither clear grouping nor outlier in the first principal plan, the green point appearing outside the ellipse being not a strong outlier according to Hotelling’s T2 range. (B) There is a clear between-group discrimination in the OPLS-DA plot along the predictive latent variable (p LV). Legend: PC1,2: Principal Components 1 and 2; o LV: first orthogonal latent variable; p LV: predictive latent variable.