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. 2019 Apr 15;10:1760. doi: 10.1038/s41467-019-09748-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Endogenous Pdgfrα promoter activity in the mammary gland. a A mouse reporter for endogenous Pdgfrα promoter activity. b Co-staining for H2B-eGFP and PDGFRα in the adult gland (representative of n = 3 mice and 5 fields per tissue section); scale bar = 25 µm. c Flow cytometry histograms depicting H2B-eGFP⁺ cells (green) in mammary subsets relative to wild-type control (black). d Mean fluorescence intensity (MFI) of H2B-eGFP in (c) (n = 3 mice). e Droplet digital PCR analysis of Pdgfrα, K14, K18 normalized to Gapdh in FACS-sorted GFP⁺ and GFP^- cell fractions from reporter mice (isolated from n = 3 individual mice). Data represent mean ± s.e.m. *p< 0.05, ***p<0.001 (one-way ANOVA). Source data are provided as a Source Data file