Fig. 6.

Stromal to epithelial transition of mesenchymal cells in a tracing-in-transplant assay. a Stromal cell contribution to epithelia was tested by a lineage tracing-in-transplant strategy utilizing FACS-sorted wild type non-fluorescent epithelial cells which were injected into inguinal cleared fat pads of PdgfrαCreERT R26mTmG tracing mice that were later TAM induced and left untreated (n = 6 fat pads) or treated with E+P hormones (n = 6 fat pads). b Images of outgrowths representative of all transplanted fat pads (white boxed area on leftmost images shown at higher magnification on right panels) with tdTomato (red), GFP (green), PDGFRα (magenta), DAPI (blue) and EpCAM (red). c Sorted wild type epithelial cell injections into cleared fat pads of AdiponectinCreERT R26mTmG mature adipocyte lineage tracing mice, also exposed to sex hormones following TAM induction. d Representative outgrowths (n = 5) resulting from transplants in (c). Scale bar = 100 µm (left; low magnification) and 25 µm (close-ups).; arrowheads indicate GFP⁺ cells. White asterisk denotes associated Supplementary Movie 4