Table 3.
kinetics of echocardiography parameters in mice implanted with Iso pump after Scramble (Scr) or Orai3 siRNA intramyocardial injection.
| Iso mice (iso pump implantation at d0) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time of echocardio-graphy | d0 | d8 | d12 | d15 | d22 | d28 | ||||||||||||
| Injection at d8 | Scr (n = 11) | Orai3 (n = 22) | p | Scr (n = 11) | Orai3 (n = 22) | p | Scr (n = 11) | Orai3 (n = 22) | p | Scr (n = 11) | Orai3 (n = 15) | p | Scr (n = 8) | Orai3 (n = 8) | p | Scr (n = 8) | Orai3 (n = 8) | p |
| HR (bpm) | 624 ± 7 | 634 ± 3 | ns | 668 ± 11 | 652 ± 9 | ns | 606 ± 15 | 619 ± 9 | ns | 622 ± 11 | 632 ± 12 | ns | 630 ± 6 | 609 ± 8 | ns | 594 ± 29 | 618 ± 11 | ns |
| IVSd (mm) | 0.6 ± 0.01 | 0.7 ± 0.01 | ns | 1 ± 0.02 | 1 ± 0.02 | ns | 1 ± 0.06 | 0.9 ± 0.03 | ns | 1 ± 0.04 | 0.8 ± 0.03 | <0.05 | 0.9 ± 0.04 | 0.8 ± 0.03 | ns | 0.8 ± 0.04 | 0.7 ± 0.04 | ns |
| LVd (mm) | 3.6 ± 0.05 | 3.5 ± 0.04 | <0.05 | 3.4 ± 0.11 | 3.6 ± 0.07 | ns | 3.7 ± 0.19 | 3.9 ± 0.09 | ns | 3.4 ± 0.17 | 4.0 ± 0.10 | <0.05 | 3.8 ± 0.18 | 4.0 ± 0.14 | ns | 4.1 ± 0.24 | 3.8 ± 0.14 | ns |
| PWd (mm) | 0.7 ± 0.02 | 0.7 ± 0.01 | ns | 0.9 ± 0.03 | 0.9 ± 0.02 | ns | 0.9 ± 0.05 | 0.8 ± 0.02 | <0.05 | 0.9 ± 0.05 | 0.7 ± 0.04 | <0.01 | 0.8 ± 0.02 | 0.7 ± 0.03 | ns | 0.7 ± 0.05 | 0.6 ± 0.03 | ns |
| IVSs (mm) | 1.1 ± 0.02 | 1.1 ± 0.02 | ns | 1.5 ± 0.03 | 1.5 ± 0.04 | ns | 1.4 ± 0.07 | 1.4 ± 0.04 | ns | 1.5 ± 0.06 | 1.1 ± 0.05 | <0.05 | 1.4 ± 0.06 | 1.3 ± 0.04 | ns | 1.3 ± 0.06 | 1.3 ± 0.07 | ns |
| LVs (mm) | 2.0 ± 0.04 | 1.9 ± 0.02 | <0.05 | 1.8 ± 0.08 | 2.0 ± 0.08 | ns | 2.1 ± 0.19 | 2.4 ± 0.12 | ns | 1.9 ± 0.16 | 2.6 ± 0.14 | <0.01 | 2.2 ± 0.17 | 2.6 ± 0.17 | ns | 2.6 ± 0.29 | 2.4 ± 0.16 | ns |
| PWs (mm) | 1.2 ± 0.03 | 1.1 ± 0.02 | ns | 1.3 ± 0.04 | 1.4 ± 0.02 | <0.05 | 1.3 ± 0.06 | 1.2 ± 0.03 | ns | 1.3 ± 0.06 | 1.0 ± 0.05 | <0.01 | 1.2 ± 0.03 | 1.0 ± 0.04 | <0.01 | 1.0 ± 0.07 | 1.0 ± 0.06 | ns |
| h/r | 0.3 ±0.007 | 0.3 ±0.004 | ns | 0.56 ±0.018 | 0.5 ±0.017 | ns | 0.54 ±0.044 | 0.4 ±0.016 | <0.05 | 0.55 ±0.041 | 0.40 ±0.021 | <0.01 | 0.44 ±0.024 | 0.39 ±0.019 | ns | 0.38 ±0.042 | 0.36 ±0.019 | ns |
| EF (%) | 83.1 ± 0.5 | 83.5 ± 0.2 | ns | 84.6 ± 0.8 | 81.7 ± 1.6 | ns | 80.0 ± 2.6 | 75.7 ± 2.1 | ns | 82.8 ± 2.2 | 68.5 ± 3.2 | <0.05 | 79.4 ± 1.9 | 70.6 ± 3 | <0.01 | 72.7 ± 4.8 | 74.1 ± 2.7 | ns |
| FS (%) | 45.9 ± 0.5 | 46.3 ± 0.2 | ns | 47.7 ± 0.9 | 45.1 ± 1.4 | ns | 44.1 ± 2.6 | 39.6 ± 1.6 | ns | 46.5 ± 2.1 | 34.2 ± 2.2 | <0.05 | 44.6 ± 1.6 | 35.3 ± 2.4 | <0.01 | 38.3 ± 2.6 | 38.1 ± 2.4 | ns |
Two-way ANOVA followed by Sidak’s post-hoc tests.
HR, heart rate; IVSd, end-diastolic interventricular septum thickness; LVd, end-diastolic left ventricular diameter; PWd, end-diastolic posterior wall thickness; IVSs, end-systolic interventricular septum thickness; LVs, end-systolic left ventricular diameter; PWs, end-systolic posterior wall thickness; h/r, diastolic wall thickness to radius ratio; EF, ejection fraction; FS, fractional shortening.